IV Quantitative Fluorescence Microscopy Course

CNC,  Sep 24th - 28th  2018

Programme

Talks

Talk 1 | Introduction to Fluorescence Microscopy

Talk 2 | Intracellular Calcium Imaging

Talk 3 | Optical Sectioning Microscopy

Talk 4 | Multiphoton Microscopy

Talk 5 | Live cell Imaging: F-techniques

Talk 6 | High speed imaging

Talk 7 | Introduction to Image analysis with FIJI

Talk 8 | Macro Design in FIJI

Talk 9 | Deconvolution

Talk 10 | An overview of the Imaris

Talk 11 | Electron Microscopy in Biomedicine

Microscopy Hands-on

Station 1 | LSM 710 NLO

Photoactivation

Brain slices imaging

 

Station 2 | LSM 710

FRAP/FRET

Tissue slice imaging

 

Station 3 | Cell Observer Spinning Disk

Calcium imaging

Mitochondria movement

 

Station 4 | Axio Imager Z2

Synaptic staining

Tissue slice imaging

 

Station 5 | Axio Observer 3

Single molecule tracking with Quantum-dots

Multicolor imaging

Image Analysis Hands-on

Hands-on 1 | An overview of Fiji software

Hands-on 2 | Deconvolution using Huygens Software

Hands-on 3 | FRAP analysis in Fiji

Hands-on 4 | Macro design in Fiji

Hands-on 5 | Image analysis with Imaris software