III Quantitative Fluorescence Microscopy Course

CNC,  Sep 18th - 22nd  2017

Programme

Talks

Talk 1 | Fluorescence Microscopy- Basic Concepts

Talk 2 | Intracellular Calcium Imaging

Talk 3 | Cell membrane receptors tracking

Talk 4 | Optical Sectioning Microscopy

Talk 5 | Live cell Imaging: F-techniques

Talk 6 | Cell Discoverer 7: Your automated platform for live cell imaging

Talk 7 | Introduction to Image analysis with FIJI

Talk 8 | Macro Design in FIJI

Talk 9 | Deconvolution

Talk 10 | NeuronRead, an open source tool for morphometric analysis of PhC (and fluorescence) neuronal images

Talk 11 | Electron Microscopy in Biomedicine

Microscopy Hands-on

Station 1 | LSM 710 NLO

Photoactivation

Brain slices staining

 

Station 2 | LSM 800

FRAP/FRET

Synaptic staining

 

Station 3 | Cell Observer Spinning Disk

Calcium Imaging

Mitochondria movement

 

Station 4 | AxioObserver Z1

Single molecule tracking with Quantum-dots

Synaptic staining

Brain slices staining

 

Station 5 | Cell Discoverer 7

Live cell imaging in multiwell plates

3D imaging

Image Analysis Hands-on

Station 1 | Intracellular calcium analysis & Tracking of mitochondria

Station 2 | Deconvolution using Huygens Software

Station 3 | FRAP analysis

Station 4 | Macro design

Station 5 | Quantification the dynamics of membrane receptors