VI Quantitative Fluorescence Microscopy Course

November 14th - 18th, 2022

Course Preliminary Program

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Day 1 - Monday, November 14

09:15    Welcome & Presentation
09:30    Basic Concepts of optical microscopy - José Rino
10:15     Fluorescence Microscopy: Basic Concepts - Margarida Caldeira
11.00     Coffee Break
11.30     Optical Sectioning Microscopy - Luísa Cortes         
12.30     Lunch
14:00     Overview of Hands-On Sessions: Sample Preparation
15:00     Live Cell Imaging: F-techniques - José Rino
16:00     Coffee Break
16:30     Automation in Live-Cell Imaging - Soren Prag 
17:30     Tour to Microscopy Rooms

Day 2 - Tuesday, November 15

09:00   Q&A
09:30   Overview of Super-Resolution - Joana Ferreira
10:30   Coffee Break
11:00    MICROSCOPY HANDS-ON I
12:30    Lunch
14.00    Expansion Microscopy - Pedro Matos Pereira
15.00    MICROSCOPY HANDS-ON II
16:30    Coffee Break
17:00    MICROSCOPY HANDS-ON III
18:30    End

Day 3 - Wednesday, November 16

09:00  Q&A
09:15   MICROSCOPY HANDS-ON IV
10:45   Coffee Break
11:00    MICROSCOPY HANDS-ON V
12.30    Lunch
14.00    Overview of Electron Microscopy - Mónica Zuzarte
15.00    Introduction to Image Analysis - José Rino
16:00   Coffee Break
16:30    IMAGE ANALYSIS HANDS-ON I: Image Analysis with ZEN software - Soren Prag

18:00   End

Day 4 - Thursday, November 17

09:00   Q&A
09:15    IMAGE ANALYSIS HANDS-ON II: First Steps in FIJI - Luísa Cortes
10:30    Coffee Break
11:00     Macro Design in FIJI - Jorge Valero
12:30    Lunch
14.00    Whole Animal Imaging - Gabriel Martins
15:00    IMAGE ANALYSIS HANDS-ON III: Macro Design in FIJI - Jorge Valero
16:30    Coffee Break
17:00     IMAGE ANALYSIS HANDS-ON IV: Introduction to IMARIS - José Rino
18:00    End

Day 5 - Friday, November 18

09:00   Q&A
09:15    IMAGE ANALYSIS HANDS-ON V: QuPath - How to analyse big images - Luísa Cortes & Telmo Pereira
10:30   Coffee Break
11.00     What is Deconvolution? - Telmo Pereira
12.30    Lunch
14.00    IMAGE ANALYSIS HANDS-ON VI: Deconvolution using Huygens Software - Telmo Pereira
16.30    Coffee Break
17:00    Bioimaging Platforms: PPBI and Euro-bioimaging - Luísa Cortes
17:30    Final Remarks

MICROSCOPY HANDS-ON

Station 1 | LSM 980

Synaptic staining
Brain slices imaging

Station 2 | Cell Discoverer 7

Automated Live-Cell Imaging

Station 3 | Cell Observer SD

Calcium imaging
Mitochondria movement

Station 4 | LSM 710

FRAP/FRET
Tissue slice imaging

Station 5 | Axio Imager Z2

Tissue slice imaging
Multicolor imaging

IMAGE ANALYSIS HANDS-ON

Hands-On I | Image Analysis with ZEN software
Hands-On II | First Steps in Fiji software
Hands-On III | Macro design in Fiji
Hands-On IV | Image analysis with IMARIS software
Hands-On V | An overview of QuPath software
Hands-On VI | Deconvolution using Huygens Software

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